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KMID : 0811720080120040171
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Korean Journal of Physiology & Pharmacology
2008 Volume.12 No. 4 p.171 ~ p.176
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Roles of ERK and NF-?B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells
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Kang Seung-Hun
Kim Koan-Hoi
Rhim Byung-Yong
Lee Ji-Hyuk
Choi Kyung-Ha
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Abstract
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Heat shock proteins (HSPs) serve as molecular chaperones and play a role in cell protection from damage in response to stress stimuli. The aim of this article is to investigate whether HSP22 affects IL-8 expression in vascular smooth muscle cells (VSMCs), and which cellular factors are involved in the HSP-mediated IL-8 induction in that cell type in terms of mitogen activated protein kinase (MAPK) and transcription element. Exposure of aortic smooth muscle cells (AoSMCs) to HSP22 not only enhanced IL-8 release but also induced IL-8 transcript via promoter activation. HSP22 activated ERK and p38 MAPK in AoSMCs. HSP22-induced IL-8 release was inhibited by U0126, but not by SB202190. A mutation in the IL-8 promoter region at the binding site of NF-?B, but not AP-1 or C/EBP, impaired promoter activation in response to HSP22. Delivery of I?B, but not dominant negative c-Jun, lowered HSP22-induced IL-8 release from AoSMCs. These results suggest that HSP22 induces IL-8 in VSMCs via ERK1/2, and that transcription factor NF-kB may be required for the HSP22-induced IL-8 up-regulation.
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KEYWORD
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Heat shock protein, Interleukin-8, Vascular smooth muscle cell
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